273 μm, Fig. 1c). These microcarriers are porous with pore size of

20.6  5.7 μm (Fig. 1d–f). Microcarrier suspension may be stored

at 4^C for 24 h. Warm to 37^C before use.

2.3

3D FloTrix^®

Digest Solution

1. Concentrated 3D FloTrix^® Digest (R005, Beijing CytoNiche

Biotechnology Co., Ltd.) solution: Dissolve 2.5 g lyophilized

powder in 1.5 L DMEM basic medium. Sterile filter through

0.22-μm filter, store at 4^C for up to 24 h. Warm to 37^C

before use.

2. Working 3D FloTrix^® Digest solution: Dissolve 10 mg lyophi-

lized powder in 10 mL DMEM basic medium. Sterile filter

through 0.22-μm filter, store at 4^C for up to 24 h. Warm to

37^C before use.

2.4

Cell Quality

Assessment-Related

Reagents

1. Trypan blue solution: 0.4%, liquid, sterile-filtered, suitable for

cell culture.

2. Antibodies:

PE-conjugated

anti-human

CD73,

FITC-

conjugated anti-human CD90, PE-conjugated anti-human

CD105, FITC-conjugated anti-human CD14, PE-conjugated

anti-human CD19, FITC-conjugated anti-human CD34,

APC-conjugated anti-human CD45, and PE-conjugated anti-

human HLA-DR CD19.

Fig. 1 (a) A photo of bottles of 3D TableTrix^® microcarrier tablets. (b) SEM images a porous microcarrier, scale

bar ¼ 100 μm. (c) Images of a tablet dispersing in culture medium at 1 s, 5 s, and after agitation

Large-Scale Expansion of UCMSCs with Microcarrier Tablets

115